Coding
CP47His

Part:BBa_K2127004

Designed by: Mirat Sojitra   Group: iGEM16_Alberta   (2016-10-14)


CP-47-HIS TAG

CP-47-HIS TAG

The his-tagged CP47 subunit is approximately 48kDa and is a transmembrane protein. As part of the Photosystem II complex, the subunit binds chlorophyll and acts as part of the light capturing array. The subunit ligand sites allow for electron movement across the peptide to facilitate movement within the electron transfer chain from both the light capturing array and from the photolysis of water. The subunit also composes part of the manganese calcium complex for oxygen evolution. It is extremely integral to the function of the PSII complex. Also in conjunction to its functions, CP47 also forms part of the quinone channel which allows direct transfer of electrons from the D1 monomer to quinones of the electron transport chain.

Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 258
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 382
    Illegal AgeI site found at 979
    Illegal AgeI site found at 1183
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 703


[edit]
Categories
//binding/metal
//cds/enzyme
//cds/membrane
//chassis/prokaryote/cyanobacterium
Parameters
chassisSynechocystis spp. PCC 6803
familyPhotosystem II Reaction Complex
functionLight Harvesting, Oxygen Reaction Complex Stabilization
ligandsHistine, Fe(II), Chlorine, Mangenase, Calcium (II), Chlorophyll, Beta-Carotene
protein47 kDa subunit