Coding

Part:BBa_K2003012

Designed by: Tora Hävermark   Group: iGEM16_Uppsala   (2016-09-29)


Inducible Green Fluorescent Protein UnaG+Flexible Linker

The inducible green fluorescent protein UnaG is 15.6 kDa, i.e. around half the size of GFP. It has maximum absorption at 498 nm and emits light at 527 nm. UnaG fluoresces only when the heme metabolite bilirubin is added (in its unconjugated form), either to a protein solution or to bacterial cells exogenously. The flexible linker is six amino acids long, added to the C terminus, enabling fusion of UnaG to other proteins. See BioBrick page BBa_K2003010 for further details on UnaG.

Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 437
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None