Coding

Part:BBa_K1886004

Designed by: Lejian Jiang   Group: iGEM16_ZJU-China   (2016-10-06)


sll1472 ccaS

CcaS is a green light-induced sensor protein from the genome of Synechocystis sp. PCC6803. It requires PCB as the chromophore to function. PCB is made by ho1 and PcyA. Furthermore, CcaS works in conjunction with CcaR, its response regulator. This is the green light sensor used by Tabor in his multichromatic light-sensing system which composed of CcaS/CcaR and Cph8/EnvZ.

This circuit is composed of the constitutive promoter and photosensitive protein gene ccaS. ccaS protein is membrane protein. After being activated by 520-nm light, it could transfer its phosphoryl group to the intracellular protein ccaR, and this process could be inhibited by 650-nm light


Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1077
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 2723
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 285
    Illegal AgeI site found at 1741
  • 1000
    COMPATIBLE WITH RFC[1000]


Characterization

BACKGROUND

Overview

Registered Parts in our wiki is the subunit of the entire light-controlled system, each of which has no function. So I will introduce our light input part of our project after the combination of all the subunits in the page of part K1886006

RESULTS


Gel electrophoretic analysis

Fig.1 Fig.1 Gel electrophoretic analyses of PCR products




[edit]
Categories
//cds/reporter/chromoprotein
Parameters
functiongreen light receptor
protein