Coding

Part:BBa_K1857000

Designed by: Joshua Timmons   Group: iGEM15_Northeastern_Boston   (2015-09-16)


mVenus

mVenus is a Chlamydomonas reinhardtii nuclear codon-optimized fluorescent protein. A variant of YFP, mVenus excites at 514nm and excites between 520 and 550nm. It includes two introns from RBCS2 in increase expression levels.

Lauersen, Kyle., Kruse, Olaf., & Mussgnug Jan. "Targeted expression of nuclear transgenes in Chlamydomonas reinhardtii with a versatile, modular vector toolkit." Applied Microbiology Biotechnology. (2015) 99;3491-3503.

Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 158
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1189
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds/reporter/yfp
//function/reporter/fluorescence
Parameters
colorYellow