Designed by Brasil-USP team, BBa_K1819000 is a GFP coding sequence with an N-terminal linker, which is 5’-flanked by NdeI restriction site.
It allows to characterize protein expression and secretion and also may serve to other IGEM teams that would like to express a chimera C-terminally attached to GFP, which exhibits bright green fluorescence when exposed to light in the blue to ultraviolet range.
We also added GGGS amino acid twice as a spacer to avoid interaction between two consecutive proteins, allowing proper folding. This strategy was already applied to BBa_K1489002 part.
Figure 1 - Schematic representation of BBa_K1819000 insert
Figure 2 - Gel restriction analysis
We added this part as an improvement to BBa_E0040, a green fluorescente protein, for protein fusion (see Experience page) .
This part was characterized using two constitutive promoters from Anderson library (BBa_J23106 and BBa_J23117). See BBa_K1819006 and BBa_K1819007 pages for more information.
- 10COMPATIBLE WITH RFC
- 12COMPATIBLE WITH RFC
- 21COMPATIBLE WITH RFC
- 23COMPATIBLE WITH RFC
- 25COMPATIBLE WITH RFC
- 1000Illegal BsaI.rc site found at 674