Coding

Part:BBa_K180001

Designed by: Richard Wilson, Stephanie Chambers   Group: iGEM09_Southampton   (2009-10-12)

Green fluorescent protein (+LVA)

Based on the part E0040, but with an LVA degradation tail attached.


Usage and Biology

LVA tag addition to end of green fluorescent protein sequence to increase the rate of degradation, which leads to GFP expression for a shorter time than the original part, E0040. This may be of use if a user is trying to monitor GFP expression under changing environmental conditions, when it would be undesirable to have GFP continually expressed for long periods of time.


Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 644


[edit]
Categories
//cds/reporter/gfp
//chassis/prokaryote/ecoli
//function/reporter/fluorescence
Parameters
colorGreen
directionforward
emission511 nm
excitation501 nm
proteinGFPmut3b
tagLVA