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Part:BBa_K1777005

Designed by: Xuhang Li   Group: iGEM15_Fudan   (2015-09-13)

flexible linker with SV40 NLS

((natural flexible linker)-(SV40 NLS)-(artificial GS linker))2
Function Protein linker
Use in Mammalian cells
RFC standard RFC 10
Backbone pSB1C3
Submitted by Fudan 2015

This is an improved flexible linker offering around 50 Angstrom separation for fusion domains.Specially, this linker was designed with a SV40 NLS, and its codon is optimized for mammalian.So it can be well used to link different protein domains for fusion proteins locating in cell nucleus.

Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//chassis/eukaryote/human
//direction/forward
//proteindomain/linker
Parameters
chassismammalian
device_typelinker
directionforward
signalling_moleculeSV40 NLS