Composite

Part:BBa_K1758335

Designed by: Team Bielefeld-CeBiTec 2015   Group: iGEM15_Bielefeld-CeBiTec   (2015-09-09)


UTR-sfGFP controlled by T7-promoter and lead responsive promoter

lead induceble promoter under the control of T7 Promoter with mRFP for detection

Usage and Biology

Characterization was conducted on this part to determine the plausibility of using it for detection of low concentrations of lead. To this effect, we characterized expression at three different concentrations of lead and documented this on a gel as seen below. The concentrations are as follows: 0, 0.012, 0.024, 0.072 glL Pb(NO3)2.

As can be seen from the gel, no appreciable difference was observed in any of the samples. While we did not conduct further testing, preliminary data suggests that this part does not function as described.

Furthermore, it should be noted that the construct contains mRFP and not sfGFP as the name might suggest, which was confirmed by BLAST.

Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//function/sensor/metal
Parameters
None