CadA promoter with CzrA binding site
In B. subtilis CadA system works as an efflux system to take the metals out in the cell. It is normally repressed by the CzrA (formely YozA) repressor protein.
When metals such as copper, zinc and arsenic are present inside the cell, they can bind to CzrA and derepress the promoter expressing CadA. Hence metals are taken out by the efflux system.
By utilising the promoter from the CadA system, we created a metal sensor, that responds to at least these three metals.
Sequence and Features
- 10COMPATIBLE WITH RFC
- 12Illegal NheI site found at 166
- 21COMPATIBLE WITH RFC
- 23COMPATIBLE WITH RFC
- 25COMPATIBLE WITH RFC
- 1000COMPATIBLE WITH RFC