Regulatory

Part:BBa_K174017

Designed by: The Newcastle 2009 iGEM team   Group: iGEM09_Newcastle   (2009-10-14)

CadA promoter with CzrA binding site

In B. subtilis CadA system works as an efflux system to take the metals out in the cell. It is normally repressed by the CzrA (formely YozA) repressor protein.

When metals such as copper, zinc and arsenic are present inside the cell, they can bind to CzrA and derepress the promoter expressing CadA. Hence metals are taken out by the efflux system.

By utilising the promoter from the CadA system, we created a metal sensor, that responds to at least these three metals.


Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 166
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//function/sensor/metal
//promoter
Parameters
None