Coding

Part:BBa_K1740001

Designed by: Keisuke Sugahara, Momoko Saegusa   Group: iGEM15_KAIT_Japan   (2015-08-20)

Dronpa145K ORF (E. coli optimized)

Dronpa 145K is similar to wild-type Dronpa. Dronpa 145K is Dronpa 145N which changed from 145Asn to 145Lys. And it has optimized to E.coli. Dronpa 145N is displaying bright green fluorescence by forming a tetramer, but Dronpa 145K brights green by a monomer. However, Dronpa 145K like 145N switched on under 390nm light from a non-fluorescent state into a fluorescent state, and back again under 503nm light.

Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 506
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds/reporter/gfp
//function/reporter/fluorescence
Parameters
colorGreen