Coding

Part:BBa_K1734004

Designed by: Eduardo Ramírez Montiel   Group: iGEM15_Tec-Monterrey   (2015-09-18)

Nanoluc (Codon optimized for Sf9 cells)

Nluc luciferase is an enzyme that was engineered to work as a luminescent reporter weighing 19.1 kDa. This protein uses fumarizine as novel substrate to produce a high intensity glow-type luminescence. These reactions are ATP-independent, trying to obtain the maximal assay sensitivity.


Tec_Monterrey_Conf_Nanluc.jpg

This part has been confirmed by restriction analysis of the pSB1C3 vector


Reference:

[1] Promega (2015). Nanoluc Genetic Reporter Vectors. URL: http://worldwide.promega.com/products/reporter-assays-and-transfection/reporter-vectors-and-cell-lines/nanoluc-luciferase-reporters/nanoluc-genetic-reporter-vectors/


Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//function/reporter/light
Parameters
None