Coding

Part:BBa_K1732027

Designed by: Donna Lee   Group: iGEM15_Carnegie_Mellon   (2015-09-18)


GLuc-His

Gaussia Luciferase (BBa_K1732003) with 6X-His tag for easier protein purification. Sequence was codon optimized for E.coli via IDT codon optimization tools.

Contribution:

Improving the documented sequence by the iGEM IONIS (Ionis_Paris), 2016
Celia Chenebault, Camille Soucies and Benjamin Piot have condon optimized the Gaussia Luciferase sequence for E.Coli DH5-alpha and for the IDT sequence.

Here is the new sequence: BBa_K2023009

For more information on our project, visit our website http://2016.igem.org/Team:Ionis_Paris


Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 411
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//function/reporter/light
Parameters
None