Protein coding region for a fusion construct of Cre Recombinase (Part:BBa_K1680007) and 145N Dronpa (Part:BBa_K1680006) with intermediate 15 amino acid linker (Part:BBa_K1680003). Oligomerisation of Dronpa (Tetramer) should stop Cre from accessing DNA in the nucleus thereby inhibiting it until light deactivation of Dronpa by illuminating with 488nm light. Dronpa can be reactivated by illumination with 405nm light.
This Part is cloned and shipped in the pTUM104 (Part:BBa_K801004) and thereby galactose inducible in yeast.
Sequence and Features
- 10COMPATIBLE WITH RFC
- 12COMPATIBLE WITH RFC
- 21Illegal BglII site found at 1381
- 23COMPATIBLE WITH RFC
- 25COMPATIBLE WITH RFC
- 1000Illegal BsaI site found at 476