Coding

Part:BBa_K1680017

Designed by: Nicolai von Kügelgen   Group: iGEM15_Tuebingen   (2015-09-17)

Cre-Dronpa Fusion

Protein coding region for a fusion construct of Cre Recombinase (Part:BBa_K1680007) and 145N Dronpa (Part:BBa_K1680006) with intermediate 6 amino acid linker (Part:BBa_K1680000). Oligomerisation of Dronpa (Tetramer) should stop Cre from accessing DNA in the nucleus thereby inhibiting it until light deactivation of Dronpa by illuminating with 488nm light. Dronpa can be reactivated by illumination with 405nm light.

This Part is cloned and shipped in the pTUM104 (Part:BBa_K801004) and thereby galactose inducible in yeast.

Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1327
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 449


[edit]
Categories
//cds
//cds/reporter/chromoprotein
//cds/reporter/gfp
//chassis/eukaryote/yeast
//function/recombination/cre
//function/reporter/fluorescence
Parameters
colorGreen