Micrococcal Nuclease (DNase)
This part contains the sequence for the Staphylococcus aureus-derived enzyme, micrococcal DNase (also known as staphylococcal nuclease or thermonuclease).
Micrococcal DNase is an endo-exonuclease that non-specifically catalyzes the hydrolysis of single- and double-stranded DNA under basic conditions and in the presence of Ca2+ ions, and is known to be able to speed up DNA hydrolysis by up to 1016 times .
The crystal structure of micrococcal DNase was resolved in 1971, long before the endogeneous function of said enzyme was discovered . As the enzyme's relatively simple structure proved to be very helpful towards the study of its biochemical and physical studies, researchers rapidly went on to identify the gene responsible for its synthesis and clone said gene in different expression hosts for heterologous characterization . Micrococcal DNase test agars are also a well-known indicator of S. aureus contamination .
It is a well-established fact that extracellular DNA is a vital structural component of bacterial biofilms, with aerosols of human recombinant DNase I having been employed as a remedy for P. aeruginosa biofilms in cystic fibrosis for two decades now . Recently, micrococcal DNase has been shown to be able to inhibit the formation of bacterial biofilms .
We are interested in the antibiofilm activity of micrococcal DNase against the biofilms formed by antibiotic-resistant strains of E. coli and P. aeruginosa found in urinary tract infections. However, in the interest of lab usage safety, for our wet lab work we will only test the antibiofilm potency of micrococcal DNase against Biosafety Level 1 laboratory strains of E. coli and P. putida.
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