RBS

Part:BBa_K1638017

Designed by: Jens Sivkśr Pettersen   Group: iGEM15_SDU-Denmark   (2015-08-12)

RBS from pKT25 and pUT18C with consensus Shine-Dalgarno

This RBS is similar to the RBS derived from pKT25 and pUT18C (BBa_K1638001), but contains the consensus Shine-Dalgarno sequence (AGGAGG) for maximal translation [1].

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

References

[1]: Karimova G, Pidoux J, Ullmann A, Ladant D. A bacterial two-hybrid system based on a reconstituted signal transduction pathway. Proceedings of the National Academy of Sciences of the United States of America. 1998;95(10):5752-6.


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