Plac -rbs- Barnase -rbs- GFP -d.term
Barnase and GFP is co-expressed by IPTG induction. This is composite parts of BBa_K1631000 which is improvement of BBa_I76211.
If you use this part, you can estimated the expression level of barnase and evaluate its toxic activity correspond to its expression level.
Usage and Biology
We succeeded to control growth rate of E coli.
We induced this parts and [Plac -rbs- GFP -d.term] with 100μM IPTG. [Plac -rbs- GFP -d.term] is negative control. We measured OD600 of 3 samples for each condition.
We also evaluated the growth rate by slope of next graph.
Plac GFP- 0.0213(/min)
Plac GFP+ 0.0205(/min)
Plac Barnase GFP- 0.0222(/min)
Plac Barnase GFP+ 0.0117(/min) Sequence and Features
- 10COMPATIBLE WITH RFC
- 12COMPATIBLE WITH RFC
- 21COMPATIBLE WITH RFC
- 23COMPATIBLE WITH RFC
- 25COMPATIBLE WITH RFC
- 1000Illegal BsaI.rc site found at 1081