Coding
HisTag-GFP

Part:BBa_K1602021

Designed by: Jasmin Weber, Michelle Zoeller, Marcus Geissler, Stefan Bohn, Simon Linder, Sebastian Barthel   Group: iGEM15_TU_Darmstadt   (2015-09-16)

Green fluorescent protein (GFP) with N-terminal His-Tag

This part encodes for the green fluorescent protein (GFP) . The sequence of a Histag (BBa_K1162009) is fused to the gene sequence, improving the purification yield.


Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 662


[edit]
Categories
//cds/reporter/gfp
//function/reporter/fluorescence
Parameters
colorGreen