Device

Part:BBa_K1554005

Designed by: Valencia_UPV team members   Group: iGEM14_Valencia_UPV   (2014-09-29)

Blue biosafety module for plants

This biosafety device for plants consists in two submodules: a male sterility submodule and an identity preservation submodule. The male sterility submodule consists in a barnase specifically expressed in anthers under the regulation of the TA29 tapetum-specific promoter. As result, pollen from these plants is not fertile. The identity preservation submodule expresses the blue chromoprotein AmilCP, so that plants can be visually differentiated from non-transgenic plants.


Usage and Biology

Our team developed a biosafety module which will be available for teams working with plants in future iGEM editions. The aim of the biosafety module is to avoid the dispersion of the plant’s genetic material in the environment. Two strategies were combined into the same module to make our plant safe: identity preservation and male sterility.

Identity preservation enables an easy identification of the genetically modified organism. A chromoprotein, which provides a differential pigmentation to the plant, is incorporated in the module. Male sterility makes impossible the dispersion of genetic material using pollen as the vehicle. In order to achieve this dispersion restriction, the gene coding for barnase (a ribonuclease from Bacillus amyloliquefaciens) under the regulation of the tapetum-specific promoter TA29 is incorporated in our system. Both components are very well documented since TA29 has been widely used and barnase has also been used under the regulation of different promoters.


Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 3097
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//biosafety
//chassis/eukaryote/nbenthamiana
Parameters
None