LacRS + MntH
MntH gene under control of Lac Repressor System
Organism: Escherichia Coli
Protein: MntH transporter from NRAMP family
Function: NRAMP is a membrane divalent metal-ion transporter. In addition to iron and manganese, it also transports Cd ions into the cell.
Aim: We want to use the endogenous transporter NRAMP to take up the cadmium from the environment. The cadmium will then activate pYoda and set off the phytochelatin synthesis process.
Expression: We can run assays measuring the concentration of cadmium in the environment. If MntH is active, the concentration of cadmium should decrease. Mutant strains: Information about mutant E. coli strains for MntH can be found at EchoBase website from the University of York (http://www.york.ac.uk/res/thomas/Gene.cfm?recordID=EB3909).
Sequence and Features
- 10COMPATIBLE WITH RFC
- 12Illegal NheI site found at 7
Illegal NheI site found at 30
- 21Illegal BglII site found at 1188
Illegal BamHI site found at 2462
- 23COMPATIBLE WITH RFC
- 25Illegal NgoMIV site found at 2358
- 1000COMPATIBLE WITH RFC