Composite

Part:BBa_K1526007

Designed by: Cauă Westmann   Group: iGEM14_York   (2014-10-07)

LacRS + MntH

MntH gene under control of Lac Repressor System

Gene: mntH
Organism: Escherichia Coli
Protein: MntH transporter from NRAMP family
Function: NRAMP is a membrane divalent metal-ion transporter. In addition to iron and manganese, it also transports Cd ions into the cell.
Aim: We want to use the endogenous transporter NRAMP to take up the cadmium from the environment. The cadmium will then activate pYoda and set off the phytochelatin synthesis process.
Expression: We can run assays measuring the concentration of cadmium in the environment. If MntH is active, the concentration of cadmium should decrease. Mutant strains: Information about mutant E. coli strains for MntH can be found at EchoBase website from the University of York (http://www.york.ac.uk/res/thomas/Gene.cfm?recordID=EB3909).


Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1188
    Illegal BamHI site found at 2462
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2358
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//binding/metal
Parameters
None