Translational_Unit

Part:BBa_K1505001

Designed by: Renyao Wei   Group: iGEM14_Penn   (2014-10-10)

RBS+smtA+mCherry

The RBS is from pmsp3 promoter, the strongest promoter isolated from Magnetospirillum bacteria. SmtA is a metallithionein isolated from E.coli and it binds covalently to divalent metal cation. It can usually binds 3-4 ions per protein, depending on the ion types. It is often used to increase the accumulation of metal ions intracellularly and potentially improve bacteria's tolerance of metal ions. The SmtA protein and mCherry protein are linked by a fusion protein linker. The purpose of the linkage is to improve the expression/folding of SmtA and for better visualization of successful transformation. The original use is to express SmtA in Magnetospirillum magneticum strain AMB-1. THis part has been sequence verified by the 2014 Penn iGEM Team.

Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 7
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//binding/metal
Parameters
None