Terminator

Part:BBa_K1486025

Designed by: EPFL iGem team 2014   Group: iGEM14_EPF_Lausanne   (2014-09-26)

Terminator.png

biology-NA-
chassisSaccharomyces Cerevisiae
directionForward
functionTerminator

ADH1 Terminator

The ADH1 terminator (Alcohol dehydrogenase) is a constitutive terminator in the budding yeast S.cerevisae.

Purpose and origin of the Biobrick

The ADH1 terminator was extracted by PCR from plasmids we ordered to addgene:Plasmid 44901 pFA6a-link-yoSuperfolderGFP-Kan and Plasmid 44873 pFA6a-link-yoSuperfolderGFP-CaURA3. A transcription terminator is necessary to avoid mRNA synthesis further than the gene of interest and control which sequence will serve as a 3'UTR, a regulatory subunit of the mRNA.

Secondary Structure

File:Mfold-K1486025-1.png


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Measurement

[edit]
Categories
//chassis/eukaryote/yeast
//direction/forward
//terminator
//terminator/double
Parameters
biology
chassisSaccharomyces Cerevisiae
directionForward
functionTerminator