Regulatory

Part:BBa_K1484214

Designed by: Salil Bhate   Group: iGEM14_Cambridge-JIC   (2014-10-08)

MpEF1a (Marchantia poylmorpha Elongation Factor 1, PlantSyntax Pro+5U)

Marchantia polymorpha Elongation Factor 1. An endogenous constitutive promoter + 5'UTR, active in meristematic tissue regions. Can be used with PlantSyntax compatible assembly techniques (RFC105)


Usage and Biology

NOTE: This part has had flanking restriction sites added to enable it to be used with type IIS assemblies compatible with PlantSyntax RFC105

MpEF1a is an important constitutive promoter for the plant chassis Marpo.

Active in meristematic tissues, as shown in the diagram below. As it is active in the earliest stages of development, this promoter can yield to high efficiencies of transformation

Time frames: This promoter is constitutive, but its expression is weak in nonmeristematic regions of the marchantia thallus:

If screening or selecting transformed Marchantia, it is recommended to use this promoter, since visible expression levels are usually observed earlier than using, for example, CaMV35S.

Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 146
    Illegal BglII site found at 234
    Illegal XhoI site found at 1479
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 858
    Illegal NgoMIV site found at 922
    Illegal NgoMIV site found at 1064
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1
    Illegal BsaI.rc site found at 1751


[edit]
Categories
//chassis/eukaryote/mpolymorpha
//promoter
//regulation/constitutive
Parameters
None