Coding

Part:BBa_K1484001

Designed by: Virginie Rutten   Group: iGEM14_Cambridge-JIC   (2014-09-02)

tsPurple chromoprotein in GoldenGate standard


Biology & Uses:
tsPurple is a chromoprotein which takes its name from TinselPurple. It exhibits a strong purple colour when expressed. The protein has its maximum absorption at ***nm giving it a purple hue visible to the naked eye. (visible wavelength: 390 to 700nm) tsPurple is a useful reporter protein and does not require microscope facilities to see.


Expression:
The chromoprotein can be readily observed when expressed in E.coli in both LB or agar plate cultures within 24h of inoculation. This protein has also been successfully expressed in Marchantia polymorpha, a promising new plant chassis. To find out more about Marchantia polymorpha and its introduction as model organism for plant synthetic biology visit Cambridge iGEM 2014 page or marchantia.org .


GoldenGate Standards:
This version of tsPurple is compatible with the GoldenGate standards as described in the 'design section' which allows more efficient assembly of parts. The protein structure or expression pattern is unchanged by this alteration which only affects the sequence before and after the CDS.


Origin:
The protein was sourced from the pre-existing biobrick: BBa_K1033906


Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1
    Illegal BsaI.rc site found at 704


[edit]
Categories
//cds/reporter/chromoprotein
//chassis/eukaryote/mpolymorpha
//chassis/prokaryote/ecoli
Parameters
None