Coding

Part:BBa_K1471001

Designed by: Jaime Antonio del Castillo Nuñez   Group: iGEM14_Biosint_MEXICO   (2014-10-08)

MerB.

Since the project is about phytoremediation, we did codon optimization of merB for its expression in Arabidopsis thaliana.


Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 411
    Illegal AgeI site found at 358
  • 1000
    COMPATIBLE WITH RFC[1000]


MerB belongs to the mercury resistant bacteria operon, is considered a key enzyme for the bioremediation and detoxification of various mercury compounds; being methylmercury the most notable and relevant. This gene codes for organomercury lyase, catalyzes the protonolysis of the mercury and carbon bond and release less toxic mercury specie (Hg2+).

Merb.png

The enzyme has two conserved cysteines residue, Cys-96 and Cys-159, which behave as a substrate binding region. This region has an important role in the cleavage of the carbon-mercury bond. The Asp-99 residue of merB plays an active function in the transference of the proton during the protonolysis. Cys-117 plays the most important structural role.

Other mechanisms have been proposed for the MerB function. One is the mechanism I, this declares that the methylmercury binds first to one of the two cysteines residues, the other cysteine will donate the proton of the leaving group (CH3) in the Hg-C bond cleavage. The mechanism II also refers that the methylmercury binds to one of the cysteines, however the other cysteine transfer the proton to the Asp99. This step allows to the cysteines to coordinate with the methylmercury, then the Asp99 protonates the CH3 and yields the Hg-C cleavage products.

Merbb.png

References

Comba P. (2011). Modeling of Molecular Properties. Weinheim,Germany:Wiley-VCH. Pages 313-320 Bizily S., Rugh C., Summers A., Meagher R. (1999). Modeling of Molecular Properties. University of Georgia, USA.

[edit]
Categories
//binding/metal
//proteindomain/degradation
Parameters
protein
proteins
protein_in