For mercury bioaccumulation.
Brief description merE
Our part is a yeast RBS with an 8KDa CH3Hg or Hg+2 transmembrane bacterial transporter.
MerE is a gene is part of the mer operon, a collection of bacterial genes specialized on the tolerance to various compounds of mercury including methylmercury. It is naturally found in the transposon Tn21 from the plasmid NR1 Shigella flexneri or MB1 in the case of Bacillus megaterium. The general mechanism of the operon can be observed on the following representation (Das S., Dash H. R., 2012):
Schematic presentation of mer operon in narrow-spectrum Gram-negative mercury-resistant bacteria (Das S., Dash H. R., 2012) Where organomercury compounds are transported inside the bacteria by merP, merT, mer E and merG, followed by the transformation of organic mercury by merB into its ionic form and the reduction from Hg+2 into volatile Hg0 by mer A with the help of NADPH(Das S., Dash H. R., 2012). Although the transportation of methylmercury is barely understood, there is evidence that recombinant E. coli and other transformed GRAM negative bacteria are able to accumulate mercury thanks to the transformation of organic mercury into its ionic form(Das S., Dash H. R., 2012).
merE has been characterized previously on A. thaliana as a potential mercury accumulator and transporter. In Kyono,M., et al (2013)¬īs study shoot and root growth were observed to become more tolerant in transgenic Arabidopsis compared with controls. As it can be seen on the figure:
Sequence and Features
- 10COMPATIBLE WITH RFC
- 12COMPATIBLE WITH RFC
- 21COMPATIBLE WITH RFC
- 23COMPATIBLE WITH RFC
- 25COMPATIBLE WITH RFC
- 1000COMPATIBLE WITH RFC
Das S., Dash H. R., (2012). Bioremediation of mercury and the importance of bacterial mer genes. National Institute of Technology.India: International Biodeterioration & Biodegradation. Volume 75. Pages 207-213
Kiyono M. , et al (2013) Increase methylmercury accumulation in Arabidopsis thaliana expressing bacterial broad-spectrum mercury transporter MerE. Springer. Issue 3; Pages 1-13
Kiyono M., Sone Y., Nakamura R., et al (2013) Role of MerC, MerE, MerF, MerT, and/or MerP in Resistance to Mercurials and the Transport of Mercurials in Escherichia coli. Biological and Pharmaceutical Bulletin. Volume 36; Issue 11; pages 1835-1841