Coding

Part:BBa_K147002

Designed by: Nathan Puhl   Group: iGEM08_University_of_Lethbridge   (2008-10-27)

xylE

The xylE gene from Pseudomonas putida encodes the enzyme catechol 2,3-dioxygenase which catalyzes the conversion of catechol to 2-hydroxymuconic semialdehyde. This reaction results in a vibrant yellow color in the cell.

Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 311
    Illegal NgoMIV site found at 483
    Illegal AgeI site found at 834
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds/enzyme
//function/reporter/color
Parameters
None