Regulatory

Part:BBa_K1467104

Designed by: Cara Deal   Group: iGEM14_NRP-UEA-Norwich   (2014-09-24)

GoldenGate compatible MAS promoter

MAS (Mannopine synthase) is a plant specific promoter which is constitutively expressed. This part is compatible with the GoldenGate MoClo Assembly Standard as it is free from internal BsaI and BpiI recognition sequences.

iGEM14_NRP-UEA-Norwich have used this part in several constructs to constitutively express the proteins AmilCP (BBa_K1467201) and AmilGFP (BBa_K1467202).

Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//chassis/eukaryote/nbenthamiana
//promoter
//regulation/constitutive
Parameters
None