Composite

Part:BBa_K1460001

Designed by: Eric Holmes   Group: iGEM14_Cornell   (2014-08-14)


pT7 + RBS + GST (glutathione-S-transferase)-CRS5 (metallothionein) + Ter

This part serves as a functional metallothionein for heavy metal binding and conferred resistance to heavy metals when combined with a strain expressing the bacteriophage T7 polymerase. CRS5 gene is the metallothionein that binds to heavy metals and it is fused to GST for stability.

Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 764
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//binding/metal
Parameters
None