Regulatory
Ph-s

Part:BBa_K143015

Designed by: Chris Hirst   Group: iGEM08_Imperial_College   (2008-09-18)

Promoter hyper-spank for B. subtilis


Promoter hyper-spank is an inducible promoter that has been designed for high expression in B.subtilis. Gene expression under the control of the promoter hyper-spank can be induced by addition of Isopropyl β-D-1-thiogalactopyranoside (IPTG). The context with which we used the promoter hyper-spank, was to take an input of IPTG and give Polymerase Per Second(PoPS) as an output. IPTG does not directly induce the expression of the promoter hyper-spank, but requires the transcriptional regulator LacI, (BBa_K143033).

LacI must be constitutively expressed in B.subtilis in order to use the promoter hyper-spank as an inducible promoter.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//rnap/prokaryote/subtilis/sigmaA
//direction/forward
//chassis/prokaryote/subtilis
//promoter
//regulation/negative
//chassis/prokaryote/Bsubtilis
Parameters
negative_regulators1
positive_regulators