Regulatory
P43

Part:BBa_K143013

Designed by: James Chappell   Group: iGEM08_Imperial_College   (2008-09-11)

Promoter 43: Constitutive Promoter for B. subtilis


Promoter 43 is a constitutive promoter that constitutively expresses the P43 protein in B.subtilis. This promoter has been shown to be recognized and active during the exponential and lag phases of growth. It has been hypothesized that the ability to recognize the promoter in exponential and lag phase of growth is due to the recognition of the promoter by both sigma factor 55 (the major sigma factor A) and sigma factor 37 (the lag phase sigma factor B) 1. The P43 promoter has been previously used for constitutive expression of exogenous genes within B.subtilis vectors 2. The context with which we used the promoter P43 is as a Polymerase Per Second (PoPS) generator.


Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]



References

<biblio>

  1. 1 pmid=6330116
  2. 2 pmid=16000826

</biblio>


[edit]
Categories
//rnap/prokaryote/subtilis/sigmaA
//rnap/prokaryote/subtilis/sigmaB
//direction/forward
//chassis/prokaryote/subtilis
//promoter
//regulation/constitutive
//chassis/prokaryote/Bsubtilis
Parameters
negative_regulators
positive_regulators