Regulatory
Pveg

Part:BBa_K143012

Designed by: James Chappell   Group: iGEM08_Imperial_College   (2008-09-11)

Promoter veg: Constitutive Promoter for B. subtilis


Pveg is a constitutive promoter that constitutively expresses the P43 protein in B. subtilis. Pveg contains binding sites for the B. sutbilis major sigma factor, Sigma factor A 1. Pveg in B. subtilis utilises two binding sites to cause high expression of genes #2, however this Pveg is lacking the upstream site to give a medium level of gene expression. It has been noted that the sporulation master regulation factor spoOA interacts with Pveg though it is not known how #3. The context with which we used the promoter Pveg is as a Polymerase Per Second (PoPS) generator.


Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]




Part Characterisation

Please see the testing constructs BBa_K143079 and BBa_K143082 for characterisation data.

References

<biblio>

  1. 1 pmid=6181373
  2. 2 pmid=6820069
  3. 3 pmid=14651647

</biblio>


[edit]
Categories
//rnap/prokaryote/subtilis/sigmaA
//direction/forward
//chassis/prokaryote/bsubtilis
//promoter
//regulation/constitutive
Parameters
negative_regulators
positive_regulators