Intermediate

Part:BBa_K1372000

Designed by: Fabio Maschi, Sylvie Lautru   Group: iGEM14_Paris_Saclay   (2014-10-02)

salicylate inducible suppressing system

This Biobrick is the result of the assembly of two Biobrick:

- BBa_J61051 for the expression of the protein NAHR, which binds to Sal promoter (Psal), to activate the transcription in response to the inducer: salicylate (substrate consumed by E. coli bacteria over time).

- BBa_K228001: for the transcription of SupD-tRNA.

Paris Saclay BB2000.png

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 786
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 77
    Illegal NgoMIV site found at 618
    Illegal AgeI site found at 1309
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1312


Made by iGEM Paris Saclay

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Parameters
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