Plasmid_Backbone

Part:BBa_K1364021

Designed by: Jourdan Camille   Group: iGEM14_Toulouse   (2014-10-03)

Integrative plasmid for Bacillus subtilis (pSBbs4E)

This part is an empty backbone vector for the usage in Bacillus subtilis. It integrates in the thrC locus and can be selected with Erythromycin . It has an Ampicillin resistance for cloning in E.coli. The backbones contains an RFP in the BioBrick site (J04450) to facilitate the cloning in coli. This backbone is origined in pKL190.

We did not observe other PstI restriction sites than the on in the sufix. We did not sequence this backbone but we suspect that it was a mistake in proprosed sequence.

Usage and Biology

Sequence and Features

Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 523
    Illegal XbaI site found at 2768
    Illegal SpeI site found at 2864
    Illegal PstI site found at 2623
    Illegal PstI site found at 4219
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 523
    Illegal EcoRI site found at 5739
    Illegal SpeI site found at 2
    Illegal SpeI site found at 2864
    Illegal PstI site found at 16
    Illegal PstI site found at 2623
    Illegal PstI site found at 4219
    Illegal NotI site found at 9
    Illegal NotI site found at 5745
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 523
    Illegal EcoRI site found at 5739
    Illegal BglII site found at 2751
    Illegal BamHI site found at 5723
    Illegal XhoI site found at 3118
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 5739
    Illegal suffix found at 2
    Illegal EcoRI site found at 523
    Illegal XbaI site found at 2768
    Illegal SpeI site found at 2864
    Illegal PstI site found at 2623
    Illegal PstI site found at 4219
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 5739
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 523
    Illegal XbaI site found at 2768
    Illegal XbaI site found at 5754
    Illegal SpeI site found at 2
    Illegal SpeI site found at 2864
    Illegal PstI site found at 16
    Illegal PstI site found at 2623
    Illegal PstI site found at 4219
    Illegal NgoMIV site found at 3082
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI.rc site found at 4045
    Illegal EcoRI site found at 523
    Illegal EcoRI site found at 5739
    Illegal XbaI site found at 2768
    Illegal XbaI site found at 5754
    Illegal SpeI site found at 2
    Illegal SpeI site found at 2864
    Illegal PstI site found at 16
    Illegal PstI site found at 2623
    Illegal PstI site found at 4219


[edit]
Categories
//chassis/prokaryote/bsubtilis
//plasmidbackbone
Parameters
None