LMU Bacillus RBS collection 2
This part was generated in RFC10, and has the following prefix and suffix:
Prefix Suffix 5' - GAATTC GCGGCCGC T TCTAGA G agaggaggata T ACTAGT A GCGGCCG CTGCAG - 3' EcoRI NotI XbaI SpeI NotI PstI
This part is used in the 2014 LMU-Munich iGEM project BaKillus.
This library was generated utilizing the Salis lab RBS calculator to find RBS that cover a high space of translation intitiation rate in 'B. subtilis'. It includes seven RBS with the following sequences:
The coverage of RBS strength as calculated by the RBS calculator is depicted in the Figure 1 shown below. It shows an evenly distributed strength spanning approxiamately three orders of magnitude.
Sequence and Features
- 10COMPATIBLE WITH RFC
- 12COMPATIBLE WITH RFC
- 21COMPATIBLE WITH RFC
- 23COMPATIBLE WITH RFC
- 25COMPATIBLE WITH RFC
- 1000COMPATIBLE WITH RFC