Signal peptide of B. subtilis minor autolysin LytE
Signal peptide (amino acids 1 to 25) of Bacillus subtilis minor autolysin LytE (probable peptidoglycan endopeptidase, Uniprot P54421). Targets proteins for secretion via the Sec pathway (Tjalsma et. al., 2000).
This part was generated in a modified version of RFC25, where a strong Shine Dalgarno Sequence (SD) is included, and has the following prefix and suffix:
|prefix with EcoRI, NotI, XbaI, SD and NgoMIV:||GAATTCGCGGCCGCTTCTAGAGTAAGGAGGAGCCGGC|
|suffix with AgeI, SpeI, NotI and PstI:||ACCGGTTAATACTAGTAGCGGCCGCTGCAG|
Sites of restriction enzymes generating compatible overhangs have the same color:
EcoRI and PstI in blue, NotI in green, XbaI and SpeI in red, NgoMIV and AgeI in orange. Shine-Dalgarno sequence and stop codons are underlined.
This part is used in the 2014 LMU-Munich iGEM project BaKillus.
Sequence and Features
- 10COMPATIBLE WITH RFC
- 12COMPATIBLE WITH RFC
- 21COMPATIBLE WITH RFC
- 23COMPATIBLE WITH RFC
- 25COMPATIBLE WITH RFC
- 1000COMPATIBLE WITH RFC