Composite

Part:BBa_K1334005

Designed by: Haoyuan Sun   Group: iGEM14_WHU-China   (2014-09-27)

P-formaldehyde+RBS+GFP+terminator

This part has been improved. The improved version is BBa_K2310901.

The promoter is activited by formaldehyde and the downstream GFP will be expressed.


Usage and Biology

We incubated E.coli strain DH5a with this part and stimulated the experimental group with 1mM formaldehyde when the OD600 value reached approximately one, which means E.coli comes into mid-log phase. And then we collected bacteria at certain time to measure the fluorescence and the absorbance.

From the Figure 1, we can see significant increase in relative fluorescence intensity after formaldehyde stimulation, which shows that our coloration system works well.

The Growth Condition curve in Figure 2 shows that the difference of the fluorescence/absorbance is not generated by the absorbance difference.

Figure 4 The function test of the formaldehyde promoter which shows our part BBa K1334002 works well 2.png

Figure 1 The function test of the formaldehyde promoter which shows our part BBa_K1334002 works well。

Figure 5 The growth condition of E.coli strain DH5a after formaldehyde stimulation.png

Figure 2 The growth condition of E.coli strain DH5a after formaldehyde stimulation.


Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1265


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