Coding

Part:BBa_K1321152

Designed by: Gabriella Santosa   Group: iGEM14_Imperial   (2014-10-09)

metallothionein Fmt fused to CBDcex

Metallothionein fMT (metal-binding peptide) fused N-terminally to CBDcex (a cellulose-binding domain).

This construct is part of a library of fusions with cellulose binding domains which we designed to bind to cellulose and enable capture of heavy metals (project page). Other fusion parts with this metal binding protein can be seen in the table below:
IC14-fMT-part-table.PNG

Note that the start and stop codon, plus 6 bp either side of the sequence, are included the RFC25 prefix and suffix which is not shown.

For reference, the CBDcex binding affinity to bacterial cellulose has been characterised relative to other sfGFP-CBD fusions, please see BBa_K1321342 for details.

Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//binding/cellulose
Parameters
None