Coding

Part:BBa_K1321091

Designed by: Xenia Spencer-Milnes   Group: iGEM14_Imperial   (2014-10-07)

Phytochelatin (PC) EC20 fused to CBDclos

Synthetic phytochelatin EC20 (metal binding peptide) fused N-terminally to CBDclos (a cellulose-binding domain)

This construct is part of a library of fusions with cellulose binding domains which we designed to bind to cellulose and enable capture of heavy metals (project page). Other fusion parts with this metal binding protein can be seen in the table below: IC14-PC-part-table.PNG

Note that the start and stop codon, plus 6 bp either side of the sequence, are included the RFC25 prefix and suffix which is not shown.

Usage and Biology

For reference, the cellulose binding domain binding capability of CBDclos (C-terminally fused) to bacterial cellulose was measured relative to other cellulose binding domains when fused to sfGFP, the data for which can be seen (here) - K1321341.

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 4
    Illegal AgeI site found at 133
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//binding/cellulose
Parameters
None