Phytochelatin (PC) EC20 fused to CBDclos
Synthetic phytochelatin EC20 (metal binding peptide) fused N-terminally to CBDclos (a cellulose-binding domain)
This construct is part of a library of fusions with cellulose binding domains which we designed to bind to cellulose and enable capture of heavy metals (project page). Other fusion parts with this metal binding protein can be seen in the table below:
Note that the start and stop codon, plus 6 bp either side of the sequence, are included the RFC25 prefix and suffix which is not shown.
Usage and Biology
For reference, the cellulose binding domain binding capability of CBDclos (C-terminally fused) to bacterial cellulose was measured relative to other cellulose binding domains when fused to sfGFP, the data for which can be seen (here) - K1321341.
- 10COMPATIBLE WITH RFC
- 12COMPATIBLE WITH RFC
- 21COMPATIBLE WITH RFC
- 23COMPATIBLE WITH RFC
- 25Illegal NgoMIV site found at 4
Illegal AgeI site found at 133
- 1000COMPATIBLE WITH RFC