Designed by: TU Munich 2013 Group: iGEM13_TU-Munich (2013-09-16)
TMD of SERK Receptor from Physcomitrella patens with C-terminal GFP fusion in RFC
This part codes for the Transmembrane Domain of SERK Receptor from Physcomitrella patens with a C-terminal (intracellular) GFPmut1 fusion; SERK TMD and GFPmut1 are seperated by a middle long linker. Still, a signal peptide is needed for membrane integration. This part is flanked by RFC pre- and suffix for further protein fusions.
Nucleotide sequence in RFC 25, so ATGGCCGGC and ACCGGT were added (in italics) to the 5' and 3' ends: (underlined part encodes the protein) ATGGCCGGCCCTGGACAA ... CTCTACAAAACCGGT ORF from nucleotide position -8 to 939 (excluding stop-codon)
Amino acid sequence: (RFC 25 scars in shown in bold, other sequence features underlined; both given below)