Designed by: Dong-Jiunn Jeffery TRUONG Group: iGEM13_TU-Munich (2013-09-16)
TMD with N-terminal Signal Peptide of SERK Receptor from ''P. patens'' in RFC N-Part
This part codes for the Transmembrane Domain with N-terminal Signal Peptide of SERK Receptor from P. patens in RFC. This construct is flanked by RFC N-part prefix and suffix. Note: This means only protein fusions to the C-terminus of this part is possible, adding promoters (typically RFC) into the prefix or terminators/IRES (typically RFC) into the suffix of this part is nevertheless possible.
Nucleotide sequence in RFC 25 N-Part using the stop codon in the suffix, so ACCGGT was added (in italics) to the 3' end: (underlined part encodes the protein) AACAATGCCTGGT ... ATTGAGGCTACCGGT ORF from nucleotide position 5 to 298 (excluding stop-codon)
Amino acid sequence: (RFC 25 scars in shown in bold, other sequence features underlined; both given below)