Designed by: Dong-Jiunn Jeffery TRUONG Group: iGEM13_TU-Munich (2013-09-17)
Secretory SpyTag (IgKappa-SigP_SpyTag) in RFC N-Part
This part encodes the SpyTag BBa_K1159201 with a N-terminal signal peptide for secretion (Ig Kappa signal peptide from Mus musculus) which also works in moss Physcomitrella patens. This construct is flanked by RFC N-part prefix and suffix. Note: This means only protein fusions to the C-terminus of this part is possible, adding promoters (typically RFC) into the prefix or terminators/IRES (typically RFC) into the suffix of this part is nevertheless possible.
Nucleotide sequence in RFC 25 N-Part using the stop codon in the suffix, so ACCGGT was added (in italics) to the 3' end: (underlined part encodes the protein) AACAATGGAAACC ... CCAACTAAGACCGGT ORF from nucleotide position 5 to 118 (excluding stop-codon)
Amino acid sequence: (RFC 25 scars in shown in bold, other sequence features underlined; both given below)