Regulatory

Part:BBa_K113009

Designed by: Zheng Wei, Wang Jinyu   Group: iGEM08_HKUSTers   (2008-10-26)

pBad/araC

It is the same as BBa_I0050. But the part seems not working so we make a new one, which the template is from iHKU team.

pBad is a promoter that is tightly controlled by an inducer (L-arabinose) and a repressor (araC). We use it to tightly control the expression of T7 polymerase as in our project the amount of T7 polymerase is essential. (BBa_K113007)

Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//rnap/prokaryote/ecoli/sigma70
//direction/forward
//chassis/prokaryote/ecoli
//promoter
//regulation/negative
Parameters
negative_regulators1
positive_regulators