Coding

Part:BBa_K1122702

Designed by: Harry Thornton   Group: iGEM13_Edinburgh   (2013-09-22)

Ferric ion-binding protein (FbpA)

This parts encodes a protein capable of binding ferric ion. The protein includes a signal peptide targeting it to periplasmic space.

Usage and Biology

FbpA was expressed using Plac promoter. Overexpression of FbpA in high iron concentration (Figure 1) gave twofold results:

-Overexpression of FbpA increases tolerance of E. coli to iron. As it can be observed on Figure 1 the cells producing the protein can grow closer to the iron source than those that do not (JM109).

-Upon closer inspection one can observe that cells overexpressing FbpA have slight red pigmentation. This could indicate that cells accumulate iron.


Fbpa functionality.jpg

Figure 1. Ferric citrate was placed on the middle of the right plate. Control cells plated on no additional iron conditions (left), cells overexpressing FbpA (plate parts lebelled FbpA1, FbpA2 and FbpA22) in high iron conditions (right). Control cells without FbpA are labelled JM109.

Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 532
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 181
    Illegal NgoMIV site found at 814
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//binding/metal
//cds
Parameters
None