Coding
SinR

Part:BBa_K1122000

Designed by: Dainius Tautvaisas   Group: iGEM13_Edinburgh   (2013-07-24)

SinR transcription factor

SinR protein is the master regulator of biofilm formation in Bacillus subtillis. In the active state, it represses biofilms. The regulatory pathway in which SinR lies is displayed in Figure 1. SinI inhibits the action of SinR by disrupting its' ability to bind DNA. The downstream regulatory network contains the exopolysaccharide (eps) and yqxM (containing an EPS bridging protein TasA) operons.

F1.medium.gif

Figure 1 The regulatory pathway of biofilm formation in Bacilus subtilis (From Winkelman et al. 2009)


Winkelman J. T., Blair K. M., Kearns D. B. (2009) RemA (YlzA) and RemB (YaaB) Regulate Extracellular Matrix Operon Expression and Biofilm Formation in Bacillus subtilis, J. Bacteriol., vol 191 no. 12, June, pp. 3981-3991.

Usage and Biology

The gene is 333bp long and can be seen clearly on a gel when cloned from B. subtilis:

AggregationResults2.png


Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//chassis/prokaryote/bsubtilis
Parameters
None