pGlnA + hmsHFRS operon
Usage and Biology
To control the expression of the operon, we used a promoter from the standard registry of parts, which was characterized by our team last year (see Part:BBa_K763003).
The hmsHFRS operon encodes 4 proteins: protein HmsH goes from 211 to 2560 bp, protein HmsF from 2561 to 597 bp, protein HmsR from 4598 to 5968 bp and protein HmsS from 5969 to 6341 bp.
The molecular mechanism works as follows: with low nitrogen in the media, the promoter is activated and a biofilm of E.coli over C.elegans should be triggered,with high nitrogen concentration, the biobrick is inactive and the bacteria will not form the biofilm.
Figure 1. Schematic operon representation.
Figure 2. Schematic representation of our biofilm-forming biobrick and its nitrogen-based regulation and how it’s seen in the NGM-PHA plates. These plates are half-made of NGM media, which is poor in nitrogen, so it induces the formation of the biofilm by activating the GlnA promoter; and half-made of PHA media, which has fatty acids that induce the Clumping and the Building of the bioplastic (PHA).
Sequence and Features
- 10COMPATIBLE WITH RFC
- 12Illegal NotI site found at 950
- 21Illegal BglII site found at 2078
Illegal BamHI site found at 5434
- 23COMPATIBLE WITH RFC
- 25Illegal NgoMIV site found at 298
Illegal NgoMIV site found at 472
Illegal NgoMIV site found at 1966
Illegal NgoMIV site found at 2374
Illegal NgoMIV site found at 2380
Illegal NgoMIV site found at 2470
Illegal NgoMIV site found at 2683
Illegal AgeI site found at 3040
- 1000Illegal SapI.rc site found at 543