Coding
sfCFP

Part:BBa_K1093002

Designed by: Anna Miścicka   Group: iGEM13_Warsaw   (2013-09-20)

sfCFP

Superfolder Cyan Fluorescent Protein is a derivative of superfolder Green Fluorescent protein. Compared to CFP it displays improved stability and strong cyan fluorescence. Excitation peak: 425 nm Emission peak: 475 nm

Team Warsaw 2013 measured sfCFP and other designed superfolder proteins and compared them to sfGFP (BBa_I746908). Measurment was conducted on pSB1A3 plasmid with J23100 promoter and B0034 RBS in living cells in RF.

caption

Fig 1. – Fluorescence of sfCFP, sfYFP and sfBFP compared to sfGFP (BBa_I746908).

- Samples Arithmetic mean Standard deviation
sfGFP 100,60 100,37 99,02 100,00 0,85
sfCFP 17,95 18,38 17,36 17,90 0,51
sfBFP 40,65 41,52 42,25 41,47 0,80
sfYFP 218,87 216,51 217,87 217,75 1,19

Tab 1. – Fluorescence of sfCFP, sfYFP and sfBFP compared to sfGFP (BBa_I746908).

References

1: Pédelacq JD, Cabantous S, Tran T, Terwilliger TC, Waldo GS. Engineering and characterization of a superfolder green fluorescent protein. Nat Biotechnol. 2006 Jan;24(1):79-88. Epub 2005 Dec 20. Erratum in: Nat Biotechnol. 2006 Sep;24(9):1170. PubMed PMID: 16369541.


Usage and Biology

sfCFP can be used as reporter.

Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds/reporter/cfp
//function/reporter/fluorescence
Parameters
colorCyan
emission475
excitation425