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Coding

Part:BBa_K1085007

Designed by: Claudio Tiecher   Group: iGEM13_Groningen   (2013-09-10)

SpiderSilkSubunitN2 Strep-tag

This BioBrick contains the coding sequence for part of a spider silk protein (SubunitN2) and a Strep-tag behind it.

SubunitN2 codes for a novel spider silk protein that is optimized for maximal expression in Bacillus subtilis 168. The primary goal of the algorithm was to optimize the codon selection based on their availability scores. The secondary goal was to minimize the number of restriction sites, and the tertiary goal was to prevent the formation of secondary RNA structures in close proximity to the RBS.

The Strep-tag, fused at the C terminal, is used both as a binding tag to use to indicate its production and as a binding component to Streptavidin.

Related BioBrick

BBa_K1085006 has the same SilkSubunitN2 but it has no Strep-tag behind it.

BBa_K1085003 has the same except it has a SpiderSilkSubunitE2 instead of SpiderSilkSubunitN2.


Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds
//chassis/prokaryote/bsubtilis
//collections/silk
Parameters
None