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Coding

Part:BBa_K1085005

Designed by: Claudio Tiecher   Group: iGEM13_Groningen   (2013-09-10)

RBS Start-codon SpiderSilkSubunitN1

This BioBrick contains the coding sequence for part of a spider silk protein (SubunitN1) with Bacillus subtilis ribosome binding site (RBS) in front of it.

The RBS is there to allow the ribosomes to bind and start to translate the DNA.

SubunitN1 codes for a navel spider silk protein that is optimized for maximal expression in Bacillus subtilis 168. The primary goal of the algorithm was to optimize the codon selection based on their availability scores. The secondary goal was to prevent the formation of secondary RNA structures in close proximity to the RBS, and the tetrary goal was to minimize the number of restriction sites.

Related BioBricks


BBa_K1085004 is the same except it has a strep-tag between Start-codon and the SilkSubunitN1 in the sequence.

BBa_K1085001 is the same except it has a SpiderSilkSubunitE1 instead of SpiderSilkSubunitN1.

Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 26
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds
//chassis/prokaryote/bsubtilis
//collections/silk
Parameters
None