Coding

Part:BBa_K1085002

Designed by: Claudio Tiecher   Group: iGEM13_Groningen   (2013-09-10)

SpiderSilkSubunitE2

This BioBrick contains the coding sequence for part of the MaSp2 spider silk protein (SubunitE2).

SubunitE2 codes for a spider silk protein that is optimized for maximal expression in Bacillus subtilis 168. The primary goal of the algorithm was to optimize the codon selection based on their availability scores. The secondary goal was to minimize the number of restriction sites, and the tertiary goal was to prevent the formation of secondary RNA structures in close proximity to the RBS.

The amino acid sequence is based on the adapted MaSp2 sequences described in the paper of Brooks et. al. 2008.


Related BioBricks

BBa_K1085003 has the same SilkSubunit but it has a Strep-tag behind it.

BBa_K1085006 has the same except it has a SpiderSilkSubunitN2 instead of SpiderSilkSubunitE2.


Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds
//chassis/prokaryote/bsubtilis
//collections/silk
Parameters
None